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Scientific Articles - PTR-MS Bibliography

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[1478] Schmidberger, T., R. Gutmann, K. Bayer, J. Kronthaler, and R. Huber, "Advanced online monitoring of cell culture off-gas using proton transfer reaction mass spectrometry", Biotechnology Progress, pp. n/a–n/a, 2014.
<p>Mass spectrometry has been frequently applied to monitor the O2 and CO2 content in the off-gas of animal cell culture fermentations. In contrast to classical mass spectrometry the proton transfer reaction mass spectrometry (PTR-MS) provides additional information of volatile organic compounds by application of a soft ionization technology. Hence, the spectra show less fragments and can more accurately assigned to particular compounds. In order to discriminate between compounds of non-metabolic and metabolic origin cell free experiments and fed-batch cultivations with a recombinant CHO cell line were conducted. As a result, in total eight volatiles showing high relevance to individual cultivation or cultivation conditions could be identified. Among the detected compounds methanethiol, with a mass-to-charge ratio of 49, qualifies as a key candidate in process monitoring due to its strong connectivity to lactate formation. Moreover, the versatile and complex data sets acquired by PTR MS provide a valuable resource for statistical modeling to predict non direct measurable parameters. Hence, partial least square regression was applied to the complete spectra of volatiles measured and important cell culture parameters such as viable cell density estimated (R2&thinsp;=&thinsp;0.86). As a whole, the results of this study clearly show that PTR-MS provides a powerful tool to improve bioprocess-monitoring for mammalian cell culture. Thus, specific volatiles emitted by cells and measured online by the PTR-MS and complex variables gained through statistical modeling will contribute to a deeper process understanding in the future and open promising perspectives to bioprocess control. &copy; 2014 American Institute of Chemical Engineers Biotechnol. Prog., 2014</p>
[Singer2011] Singer, W., J. Herbig, R. Gutmann, K. Winkler, I. Kohl, and A. Hansel, "Applications of PTR-MS in medicine and biotechnology", American Laboratory, vol. 43, no. 7: AMER LABORATORY-LABCOMPARE 30 CONTROLS DRIVE, SHELTON, CT 06484 USA, pp. 34–37, 2011.
Proton transfer reaction-mass spectrometry (PTR-MS) is a well-established analytical tool for the measurement of volatile organic compounds (VOCs), and offers real-time detection and quantification of VOCs at trace concentrations. This paper focuses on the measurement of VOCs in biological systems. Both microorganisms and cells, e.g., in the human body, constantly produce a large variety of volatile organic metabolites. Analyzing VOCs in exhaled human breath reveals information about the status of the body. In a similar manner, monitoring the off-gas of fermentations in the biopharmaceutical industry allows microbial activity to be gauged. Undesired compounds (those that are harmful to the human body or impurities in biotechnical processes) can also be tracked in real time using the technique.

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Selected PTR-MS related Reviews

F. Biasioli, C. Yeretzian, F. Gasperi, T. D. Märk: PTR-MS monitoring of VOCs and BVOCs in food science and technology, Trends in Analytical Chemistry 30 (7) (2011).

J. de Gouw, C. Warneke, T. Karl, G. Eerdekens, C. van der Veen, R. Fall: Measurement of Volatile Organic Compounds in the Earth's Atmosphere using Proton-Transfer-Reaction Mass Spectrometry. Mass Spectrometry Reviews, 26 (2007), 223-257.

W. Lindinger, A. Hansel, A. Jordan: Proton-transfer-reaction mass spectrometry (PTR–MS): on-line monitoring of volatile organic compounds at pptv levels, Chem. Soc. Rev. 27 (1998), 347-375.


Lists with PTR-MS relevant publications of the University of Innsbruck can be found here: Atmospheric and indoor air chemistry, IMR, Environmental Physics and Nano-Bio-Physics


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