The world's leading PTR-MS company

Ultra-Sensitive Real-Time Trace Gas Analyzers  •  Modular TOF-MS for Research & OEM


You are here

Scientific Articles - PTR-MS Bibliography

Welcome to the new IONICON scientific articles database!


Found 5 results
Title [ Year(Asc)]
Filters: First Letter Of Title is A and Author is Aprea, Eugenio  [Clear All Filters]
[1548] Aprea, E., L. Cappellin, F. Gasperi, F. Morisco, V. Lembo, A. Rispo, R. Tortora, P. Vitaglione, N. Caporaso, and F. Biasioli, "Application of PTR-TOF-{MS} to investigate metabolites in exhaled breath of patients affected by coeliac disease under gluten free diet", Journal of Chromatography B, vol. 966, pp. 208–213, Sep, 2014.
<p>Coeliac disease (CD) is a common chronic inflammatory disorder of the small bowel induced in genetically susceptible people by the exposure to gliadin gluten. Even though several tests are available to assist the diagnosis, CD remains a biopsy-defined disorder, thus any non-invasive or less invasive diagnostic tool may be beneficial. The analysis of volatile metabolites in exhaled breath, given its non-invasive nature, is particularly promising as a screening tool of disease in symptomatic or non-symptomatic patients. In this preliminary study the proton transfer reaction time of flight mass spectrometry coupled to a buffered end-tidal on-line sampler to investigate metabolites in the exhaled breath of patients affected by coeliac disease under a gluten free diet was applied. Both H3O+ or NO+ were used as precursor ions. In our investigation no differences were found in the exhaled breath of CD patients compared to healthy controls. In this study, 33 subjects were enrolled: 16 patients with CD, all adhering a gluten free diet, and 17 healthy controls. CD patients did not show any symptom of the disease at the time of breath analysis; thus the absence of discrimination from healthy controls was not surprising.</p>
[Aprea2012] Aprea, E., F. Morisco, F. Biasioli, P. Vitaglione, L. Cappellin, C. Soukoulis, V. Lembo, F. Gasperi, G. D'Argenio, V. Fogliano, et al., "Analysis of breath by proton transfer reaction time of flight mass spectrometry in rats with steatohepatitis induced by high-fat diet.", J Mass Spectrom, vol. 47, no. 9: IASMA Research and Innovation Centre, Fondazione Edmund Mach, Food Quality and Nutrition Department, Via E. Mach, 1, 38010, S. Michele a/A, Italy., pp. 1098–1103, Sep, 2012.
Breath testing has been largely used as a diagnostic tool, but the difficulties in data interpretation and sample collection have limited its application. We developed a fast (< 20?s), on-line, non-invasive method for the collection and analysis of exhaled breath in awake rats based on proton transfer reaction time of flight mass spectrometry (PTR-ToF-MS) and applied it to investigate possible relationships between pathologies induced by dietary regime and breath composition. As a case study, we investigated rats with dietary induced non-alcoholic steatohepatitis (NASH) and modifications induced by coffee addition to the diet. We considered two different diets (standard and high fat) complemented with two different drinking possibilities (water or decaffeinated coffee) for a total of four groups with four rats each. Several spectrometric peaks were reliable markers for both dietary fat content and coffee supplementation. The high resolution and accuracy of PTR-ToF-MS allowed the identification of related compounds such as methanol, dimethyl sulphide, dimethyl sulphone and ammonia. In conclusion, the rapid and minimally invasive breath analysis of awake rats permitted the identification of markers related to diet and specific pathologic conditions and provided a useful tool for broader metabolic investigations.
[Aprea2007a] Aprea, E., F. Biasioli, F. Gasperi, D. Mott, F. Marini, and T. D. Maerk, "Assessment of Trentingrana cheese ageing by proton transfer reaction-mass spectrometry and chemometrics", International dairy journal, vol. 17, no. 3: Elsevier, pp. 226–234, 2007.
Proton transfer reaction-mass spectrometry (PTR-MS) data have been analysed by chemometric techniques to monitor cheese ageing by means of on-line direct head-space gas analysis. Twenty cheese loaves of Trentingrana, a trademarked cheese produced in northern Italy, of different origin and ripening degree, were sampled over the whole Trentingrana production area. An increase of the spectral intensity with ripening has been observed for most of the PTR-MS peaks and a univariate analysis identified 16 mass peaks that were significantly different for ripened and young cheeses, respectively. Moreover, the usefulness of different discriminant analyses and class modelling techniques have been investigated. Discriminant Partial Least Squares analysis, while indicating average behaviour and possible outliers, was not able to correctly classify all samples. Soft class modelling performed better and allowed a 100% correct classification. Partial least square calibration predicted the ageing time of each loaf with reasonable accuracy with a maximum cross-validation error of 3.5 months.
[Aprea2005] Aprea, E., F. Biasioli, F. Gasperi, G. Sani, C. Cantini, and T. D. Maerk, "Advanced oxidation in olive oil: monitoring of secondary reaction products and detection of rancid defect", Mass Spectrometry and Its Applications, vol. -, pp. 144, 2005.
[Biasioli2004a] Biasioli, F., F. Gasperi, G. Odorizzi, E. Aprea, and D. Mott, "Applicabilità del PTR-MS al controllo degli odori negli impianti per il trattamento dei rifiuti", Rifiuti solidi, 2004.

Featured Articles

Download Contributions to the International Conference on Proton Transfer Reaction Mass Spectrometry and Its Applications:


Selected PTR-MS related Reviews

F. Biasioli, C. Yeretzian, F. Gasperi, T. D. Märk: PTR-MS monitoring of VOCs and BVOCs in food science and technology, Trends in Analytical Chemistry 30 (7) (2011).

J. de Gouw, C. Warneke, T. Karl, G. Eerdekens, C. van der Veen, R. Fall: Measurement of Volatile Organic Compounds in the Earth's Atmosphere using Proton-Transfer-Reaction Mass Spectrometry. Mass Spectrometry Reviews, 26 (2007), 223-257.

W. Lindinger, A. Hansel, A. Jordan: Proton-transfer-reaction mass spectrometry (PTR–MS): on-line monitoring of volatile organic compounds at pptv levels, Chem. Soc. Rev. 27 (1998), 347-375.


Lists with PTR-MS relevant publications of the University of Innsbruck can be found here: Atmospheric and indoor air chemistry, IMR, Environmental Physics and Nano-Bio-Physics


Download the latest version of the IONICON publication database as BibTeX.