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Scientific Articles - PTR-MS Bibliography

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Found 3 results
Title [ Year(Asc)]
Filters: Author is Lindinger, W.  [Clear All Filters]
[Karl2002a] Karl, T.., R.. Fall, T.. N. Rosenstiel, P.. Prazeller, B.. Larsen, G.. Seufert, and W.. Lindinger, "On-line analysis of the (13)CO(2) labeling of leaf isoprene suggests multiple subcellular origins of isoprene precursors.", Planta, vol. 215, no. 6: Institut fuer Ionenphysik, Universitaet Innsbruck, Technikerstrasse 25, 6020 Innsbruck Austria., pp. 894–905, Oct, 2002.
Isoprene (2-methyl-1,3-butadiene) is the most abundant biogenic hydrocarbon released from vegetation, and there is continuing interest in understanding its biosynthesis from photosynthetic precursors in leaf chloroplasts. We used on-line proton-transfer-reaction mass spectrometry (PTR-MS) to observe the kinetics of (13)C-labeling of isoprene following exposure to (13)CO(2) and then the loss of (13)C after a return to normal (12)CO(2) in oak ( Quercus agrifolia Nee) and cottonwood (Populus deltoides Barr.) leaves. Assignments of labeled isoprene species were verified by gas chromatography-mass spectrometry. For the first time, it was possible to observe the half-lives of individually (13)C-labeled isoprene species during these transitions, and to trace some of the label to a C3 fragment that contained the two isoprene carbons derived from pyruvate via the deoxyxylulose-5-phosphate (DOXP) pathway. At steady state (under (13)CO(2)), approximately 80% of isoprene carbon was labeled, with fully labeled isoprene as the major species (approx. 60%). The source of the unlabeled C is suggested to be extrachloroplastic, but not from photorespiratory carbon. After a transfer to (12)CO(2), (13)C-labeling persisted in one isoprene carbon for several hours; this persistence was much more pronounced in (i) leaves inhibited by fosmidomycin, a specific inhibitor of the DOXP pathway, and (ii) in sun leaves which have higher ratios of soluble sugars to starch. From the mass 41-44 fragment data, and labeling predicted from the DOXP pathway in chloroplasts, precursors may arise from cytosolic pyruvate/phospho enolpyruvate equivalents transported into the chloroplast; this idea was supported by an indirect measure of pyruvate labeling. Other sources of cytosolic isoprene precursors (i.e. dimethylallyl diphosphate or pentose phosphate) could not be excluded. The data obtained shed light on the half-lives of photosynthetic metabolites, exchanges of carbon between cellular pools, and suggest multiple origins of isoprene precursors in leaves.
[Rieder2001] Rieder, J.., P.. Lirk, C.. Ebenbichler, G.. Gruber, P.. Prazeller, W.. Lindinger, and A.. Amann, "Analysis of volatile organic compounds: possible applications in metabolic disorders and cancer screening.", Wien Klin Wochenschr, vol. 113, no. 5-6: Department of Anesthesiology and Critical Care Medicine, Leopold-Franzens University, Innsbruck, Austria., pp. 181–185, Mar, 2001.
The human breath contains a variety of endogenous volatile organic compounds (VOCs). The origin and pathophysiological importance of these VOCs is poorly investigated. Little is known about the interaction of VOCs from ambient air, such as those produced by plants and exhaust fumes, with the human organism. Gas chromatographic determination of VOC concentrations is tedious. Proton-transfer-mass spectroscopy (PTR-MS), a new technology for the online detection of VOC patterns, is a valuable alternative. We present two interesting molecular species, isoprene and ortho (o)-toluidine, as examples of endogenously produced VOCs. In a case study, breath isoprene reductions during lipid-lowering therapy (36%) were shown to correlate with cholesterol (32%) and LDL concentrations (35%) in blood (p < 0.001) over a period of 15 days. Therefore, isoprene concentrations in human breath (measured by PTR-MS) might serve as an additional parameter to complement invasive tests for controlling lipid-lowering therapy. Furthermore, it may be a useful parameter for lipid disorder screening. Mass-108, which presumably represents o-toluidine in our breath samples, was found in significantly higher concentrations in the breath of patients with different tumors (1.5 +/- 0.8 ppbv) than in age-matched controls (0.24 +/- 0.1 ppbv, p < 0.001). Inflammatory reactions do not seem to alter the pattern of mass-108. Therefore, it appears to be a currently underestimated carcinoma marker that deserves further investigation.
[Taucher1997] Taucher, J.., A.. Hansel, A.. Jordan, R.. Fall, J.. H. Futrell, and W.. Lindinger, "Detection of isoprene in expired air from human subjects using proton-transfer-reaction mass spectrometry.", Rapid Commun. Mass Spectrom., vol. 11, pp. 1230-4, 1997.
A new analytical method using proton-transfer-reaction mass spectrometry (PTRMS) is described for the determination of trace constituents in human breath. PTRMS is sufficiently sensitive and specific that it does not require preconcentration or separation. At its present stage of development it is capable of detecting trace constituents present in air at the part-per-billion level. These capabilities are illustrated for isoprene, one of the most abundant endogenous hydrocarbons. Our results confirm recent observations of a diurnal level variation associated with sleep or wakefulness; a new finding is that young children have much lower levels of isoprene in breath than adults. To address the metabolic origin of human isoprene, we used PTRMS to analyze expired air for allylic C5 alcohols that have been proposed to be non-enzymatic precursors of isoprene. The lack of correlation between peak breath isoprene and these alcohols suggests that the hydrocarbon is formed by some other mechanism.

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Selected PTR-MS related Reviews

F. Biasioli, C. Yeretzian, F. Gasperi, T. D. Märk: PTR-MS monitoring of VOCs and BVOCs in food science and technology, Trends in Analytical Chemistry 30 (7) (2011).

J. de Gouw, C. Warneke, T. Karl, G. Eerdekens, C. van der Veen, R. Fall: Measurement of Volatile Organic Compounds in the Earth's Atmosphere using Proton-Transfer-Reaction Mass Spectrometry. Mass Spectrometry Reviews, 26 (2007), 223-257.

W. Lindinger, A. Hansel, A. Jordan: Proton-transfer-reaction mass spectrometry (PTR–MS): on-line monitoring of volatile organic compounds at pptv levels, Chem. Soc. Rev. 27 (1998), 347-375.


Lists with PTR-MS relevant publications of the University of Innsbruck can be found here: Atmospheric and indoor air chemistry, IMR, Environmental Physics and Nano-Bio-Physics


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