[LaPorta2004] "Discrimination of Heterobasidion annosum ISGs by evaluation of volatile organic compounds",
S. Michele all'Adige, Italy
, vol. 27, no. 8, pp. 379–382, 2004.
Heterobasidion annosum represents one of the most dangerous fungi in the conifer forest of the boreal hemisphere. This fungus was differentiated into three biological species (Intersterile groups; ISGs) characterized by different host specificity, patogenicity and distribution. A fast and easy identification of the ISG has important consequences on the silvicultural decition making. However, the determination of the ISG from the morphological traits of fruit bodies or, least of all, from mycelium is usually not easy. Proton Transfer Reaction – Mass Spectrometry (PTR-MS) is a new technique proposed and realised by the University of Innsbruck, and now issued also to the market. This technique allows high performance on-line measurements for a large number of VOCs. The aim of this work was to verify the possibility to discriminate the H. annosum ISGs based on their volatile compound emission and to identify putative characteristic masses that may play an important role in the host specificity and in the specific antagonistic fungi among each ISGs. Twelve strains belong to the three ISGs (here idicated by F, P and S) were analysed by PTRMS fingerprinting. P ISG was definitely separated from F and S ISGs. In addition, several masses show to be significantly different among the three ISGs. ANOVA on PTR-MS peak values identified 16 significant masses out of 230. Applications and limitations of this approach are discussed.